3D Imaging Shows Human Sperm Roll Like Playful Otters
Imaging that reconstructs the movement of human sperm in 3D has shattered the view of how sperm “swim” first proposed 300 years ago by Antonie van Leeuwenhoek. This could be a breakthrough in fertility science by researchers from Bristol and Mexico.
van Leeuwenhoek used one of the earliest microscopes to describe human sperm as having a “tail, which, when swimming, lashes with a snakelike movement, like eels in water.” This is, however, an optical illusion.
Using state-of-the-art 3D microscopy and mathematics, Drs. Hermes Gadelha, Gabriel Corkidi and Alberto Darszon have pioneered the reconstruction of the true movement of the sperm tail in 3D.
Using a high-speed camera capable of recording over 55,000 frames in one second, and a microscope stage with a piezoelectric device to move the sample up and down at an incredibly high rate, they were able to scan the sperm swimming freely in 3D.
The ground-breaking study, published in the journal Science Advances, reveals the sperm tail is in fact wonky and only wiggles on one side. While this should mean the sperm’s one-sided stroke would have it swimming in circles, sperm have found a clever way to adapt and swim forwards.
“Human sperm figured out if they roll as they swim, much like playful otters corkscrewing through water, their one-sided stroke would average itself out, and they would swim forwards,” said Gadelha, head of the Polymaths Laboratory at Bristol’s Department of Engineering Mathematics and an expert in the mathematics of fertility.
“The sperms’ rapid and highly synchronised spinning causes an illusion when seen from above with 2D microscopes – the tail appears to have a side-to-side symmetric movement, “like eels in water”, as described by Leeuwenhoek in the 17th century.
“However, our discovery shows sperm have developed a swimming technique to compensate for their lop-sidedness and in doing so have ingeniously solved a mathematical puzzle at a microscopic scale: by creating symmetry out of asymmetry,” said Gadelha.
“The otter-like spinning of human sperm is however complex: the sperm head spins at the same time that the sperm tail rotates around the swimming direction. This is known in physics as precession, much like when the orbits of Earth and Mars precess around the sun.”
Computer-assisted semen analysis systems in use today, both in clinics and for research, still use 2D views to look at sperm movement. Therefore, like Leeuwenhoek’s first microscope, they are still prone to this illusion of symmetry while assessing semen quality. This discovery, with its novel use of 3D microscope technology combined with mathematics, may provide fresh hope for unlocking the secrets of human reproduction.
“With over half of infertility caused by male factors, understanding the human sperm tail is fundamental to developing future diagnostic tools to identify unhealthy sperm,” adds Gadelha, whose work has previously revealed the biomechanics of sperm bendiness and the precise rhythmic tendencies that characterize how a sperm moves forward.
Corkidi and Darszon pioneered the 3D microscopy for sperm swimming.
“This was an incredible surprise, and we believe our state-of-the-art 3D microscope will unveil many more hidden secrets in nature. One day this technology will become available to clinical centres,” said Dr Corkidi.
“This discovery will revolutionize our understanding of sperm motility and its impact on natural fertilization. So little is known about the intricate environment inside the female reproductive tract and how sperm swimming impinge on fertilization. These new tools open our eyes to the amazing capabilities sperm have,” said Darszon.
Source: Bristol University
